The magnetic cell separation method reveals protective effect of melatonin on human spermatozoa from peroxide-induced apoptosis

Abstract Background The selection of non-apoptotic sperm is related to successful fertilization. This study investigated the protective effects melatonin and role MACS (magnetically activated cell sorting) method prevent oxidative damage in vitro improve quality parameters such as viability DNA integrity. Materials methods Semen analysis was performed on 20 different eligible donors participating study. Sperm concentration were checked at time 0 (0 h). In order conduct more studies after 24 h (24 h), remaining suspension divided into a control group six experimental groups. fragmentation assessed using TUNEL assay. Results treatment human spermatozoa with 100 µM hydrogen peroxide for induced significant increase phosphatidylserine externalization significantly increases apoptotic ( p ≤ 0.001). pretreated showed that percentage fragmented reduced sorting by P However, 1 μM could effectively maintain motility progressive motility. Conclusions Pretreated be effective maintenance Although peroxide-treated used, used retain appropriate select high-quality sperm.